Gab1和Sfmbt2基因在成年普通牛8种不同组织中的印记状态分析

doi:10.11843/j.issn.0366-6964.2017.06.004

畜牧兽医学报 ›› 2017, Vol. 48 ›› Issue (6): 1000-1006.doi: 10.11843/j.issn.0366-6964.2017.06.004

Gab1和Sfmbt2基因在成年普通牛8种不同组织中的印记状态分析

王冠楠¹, 赵宇鹏², 陈玮娜³, 张萃¹, 徐达¹, 李冬杰⁴, 李世杰^1*

1. 河北农业大学生命科学学院, 保定 071001;
2. 河北北方学院, 张家口 075000;
3. 河北大学中医系, 保定 071001;
4. 河北科技大学生物科学与工程学院, 石家庄 050018

收稿日期:2017-01-06 出版日期:2017-06-23 发布日期:2017-06-23
通讯作者: 李世杰,博士生导师,教授,主要从事动物转基因克隆技术和动物分子遗传的研究,E-mail:lishijie20005@163.com
作者简介:王冠楠(1992-),男,河北廊坊人,硕士生,主要从事动物分子遗传学研究,Tel:0312-7528270,E-mail:wangguannan710@163.com
基金资助:
国家自然科学基金（31372312）；河北省自然科学基金（C2016204092）

Genomic Imprinting Status of Gab1 and Sfmbt2 in Different Tissues of Adult Cattle

WANG Guan-nan¹, ZHAO Yu-peng², CHEN Wei-na³, ZHANG Cui¹, XU Da¹, LI Dong-jie⁴, LI Shi-jie^1*

1. College of Life Science, Hebei Agricultural University, Baoding 071001, China;
2. Hebei North University, Zhangjiakou 075000, China;
3. Department of Traditional Chinese Medicine, Hebei University, Baoding 071001, China;
4. College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang 050018, China

Received:2017-01-06 Online:2017-06-23 Published:2017-06-23

摘要/Abstract

摘要：

旨在揭示Gab1（Grb2-associated binding protein 1）和Sfmbt2（Scm-like with four mbt domains 2）基因在牛不同组织以及胎盘中的印记状态，本研究应用基于单核苷酸多态性（Single nucleotide polymorphisms，SNP）的RT-PCR（Reverse transcription-PCR）产物直接测序方法对Gab1和Sfmbt2基因在牛7个组织（心、肝、脾、肺、肾、肌肉和脂肪）以及胎盘中的印记状态进行分析。结果显示，在被分析的7个组织和胎盘中均检测到Gab1和Sfmbt2基因的表达。通过比较杂合子牛基因组PCR扩增产物与RT-PCR扩增产物在SNP位点的测序峰图，发现Gab1和Sfmbt2在被检测的7个组织和胎盘中均为双等位基因表达，说明Gab1和Sfmbt2在牛的组织和胎盘中是非印记的。

Abstract:

In order to reveal the imprinting status of Gab1 (Grb2-associated binding protein 1) and Sfmbt2 (Scm-like with four mbt domains 2) in different tissues and placenta of bovine, in this study, we analyzed the imprinting status of Gab1 and Sfmbt2 in 7 bovine tissues (heart, liver, spleen, lung, kidney, muscle and fat) and placenta by direct sequencing the RT-PCR products based on single nucleotide polymorphisms.The result displayed that Gab1 and Sfmbt2 were all expressed in 7 tissues and placenta.We discovered that Gab1 and Sfmbt2 were bi-allelic expressed by comparing sequence chromatograms of RT-PCR products and genomic PCR products at single nucleotide polymorphisms sites, suggesting that Gab1 and Sfmbt2 were not imprinted in adult cattle tissues and placenta.

中图分类号:

S823
S813.3

王冠楠, 赵宇鹏, 陈玮娜, 张萃, 徐达, 李冬杰, 李世杰. Gab1和Sfmbt2基因在成年普通牛8种不同组织中的印记状态分析[J]. 畜牧兽医学报, 2017, 48(6): 1000-1006.

WANG Guan-nan, ZHAO Yu-peng, CHEN Wei-na, ZHANG Cui, XU Da, LI Dong-jie, LI Shi-jie. Genomic Imprinting Status of Gab1 and Sfmbt2 in Different Tissues of Adult Cattle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(6): 1000-1006.

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Gab1和Sfmbt2基因在成年普通牛8种不同组织中的印记状态分析

Genomic Imprinting Status of Gab1 and Sfmbt2 in Different Tissues of Adult Cattle

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